Effects of Didang Qigui Recipe on Islet Inflammation and Structure of PancreaticβCell in Streptozotocin-induced Diabetic Rats
10.3969/j.issn.1005-5304.2014.10.018
- VernacularTitle:抵当芪桂汤对糖尿病大鼠胰岛炎症及β细胞结构的影响
- Author:
Jingfeng YANG
;
Yanyun REN
;
Wei LENG
;
Tiancai ZHAO
;
Shuai WANG
- Publication Type:Journal Article
- Keywords:
diabetes;
Didang Qigui Recipe;
IL-1β;
IL-2;
structure of pancreaticβcell;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(10):61-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Didang Qigui Recipe on preventing diabetes. Methods Ten female rats were set randomly as normal group, and others 70 were injected with STZ (60 mg/kg) to establish the diabetic model. After the model was established, 50 model rats were randomly divided into five groups:the model group, Didang Qigui Recipe group, the gliclazide group, Didang Qigui Recipe high-dose group, Didang Qigui Recipe and gliclazide group, with 10 rats in each group. All groups were given a gavage with related medicine. With treatment being given respectively, eight weeks later, contents of IL-1βand IL–2 in serum were detected by ELISA and structure of pancreatic β cells was observed by immunohistochemistry and microscope. Results Compared with the model group, the contents of IL-1βand IL-2 in serum of rats decreased in treatment groups, especially in Didang Qigui Recipe group and Didang Qigui and gliclazide group (P<0.05). The degree of apoptosis in pancreaticβcells, especially in the above two groups, has been less affected than other groups (P<0.05). Conclusion Didang Qigui Recipe has preventive and therapeutic effects on diabetes by inhibition of inflammatory response and protection structure of pancreaticβcells.
