Suppressive effects of bezafibrate on proliferation and cytokine production by CD4+T cells from patients with primary biliary cirrhosis
10.3969/j.issn.1000-484X.2014.10.019
- VernacularTitle:苯扎贝特对原发性胆汁性肝硬化患者外周血CD4＋T细胞的抑制作用研究
- Author:
Qubo CHEN
;
Anping PENG
;
Cuicui LI
;
Rong ZHAO
;
Xinyi LU
;
Min HE
;
Limin ZHOU
;
Weilin WU
- Publication Type:Journal Article
- Keywords:
Bezafibrate;
PBC;
CD4+T cells;
Cytokines
- From:
Chinese Journal of Immunology
2014;(10):1388-1392
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of bezafibrate (BF) on the activation,proliferation and differentiation of CD4+T cells from primary biliary cirrhosis ( PBC) patients and to elucidate the mechanisms for the immunosuppressive effects of BF and to further provide experience basis for BF target therapy PBC.Methods:PBMCs were isolated from PBC patients then CD 4+T cells were selected by MACS, and stimulated with anti-CD3, anti-CD28, in the presence of different concentration of BF.The cytokines were measured by ELISA,and the activation,proliferation and differentiation of CD4+T cells were analyzed by flow cytometry.Results:(1) BF could inhibit the activation of CD 4+T cells in PBC patients.(2) BF could inhibit the proliferation of CD 4+T cells in PBC patients in a dose-dependent manner (P<0.05).(3)BF could down-regulation IFN-γand IL-17 production of CD4+T cells in a dose-dependent manner ( P<0.05 ).Conclusion: BF could inhibit immune responses of PBC patients by suppressing CD 4+T cells activation;proliferation and cytokine production.
