Direct activation of expression vector withβ-casein gene promoter using engineered TALE transcription factors in mouse fibroblasts
10.3969/j.issn.1005-4847.2014.05.003
- VernacularTitle:利用 TALE-TFs在小鼠成纤维细胞中激活β-酪蛋白基因启动子表达载体
- Author:
Wenhui PI
;
Long LIANG
;
Hong TANG
;
Yiyuan ZHANG
;
Yanhua GUO
;
Limin WANG
;
Chunhe XIANG
;
Ping ZHOU
;
Shouren LIU
- Publication Type:Journal Article
- Keywords:
Transcription activator-like effector-transcription factors (TALE-TFs);
Mouse fibroblasts;
β-casein gene promoter;
Gene expression modulation
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(5):13-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective TALE-TFs were adopted to provide a new way in detection of the expression result ofβ-ca-sein gene promoter-interesting gene expression cassettes in mouse fibroblasts.Methods TALE-TFs of eukaryotic expres-sion plasmid and expression cassette withβ-casein gene promoter and red fluorescent protein reporter gene were co-nucleo-fected into mouse fibroblasts by Amaxa nucleofector.Results and Conclusion β-casein gene promoter was activated by artificial TALE-TFs in the mouse fibroblasts.The way is a new expression verification system instead of mammary epithelial cells with fibroblasts.
