Establishment of an anti-CD45 monoclonal antibody that inhibits T-cell proliferation
10.3969/j.issn.1005-4847.2014.05.008
- VernacularTitle:抑制T细胞增殖的抗CD45单克隆抗体的建立
- Author:
Zehong SU
;
Yalin LI
;
Xiangwen TAN
;
Xin LIU
;
Yueqin YUAN
;
Gaojian LIAN
- Publication Type:Journal Article
- Keywords:
CD45;
Monoclonal antibody;
Inhibition;
T-cell proliferation;
Mouse;
Rat;
Dendritic cells
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(5):35-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify molecules that modulate T-cell functions and serve the studies on T-cell media-ted autoimmune diseases.Methods Bone marrow-derived dentritic cells were collected from BALB/c mice to immunize Wistar rats, and to establish many hybridoma cell lines.Many hybridoma cell lines which could modulate T-cell functions were obtained.One of the cell lines, most actively inhibiting T-cell proliferation, was further studied.Results The anti-CD45 mAb recognized CD45 and significantly suppressed T-cell proliferation in proliferation assays.Conclusions Our re-sults indicate that the anti-CD45 mAb can effectively suppress T-cell proliferation, and is promising to be used in the pre-vention and treatment of T-cell mediated autoimmune diseases in the future.
