Distribution of green fluorescent protein (GFP) expression in green fluorescent nude mice
10.3969/j.issn.1005-4847.2014.05.015
- VernacularTitle:GFP 在绿色荧光裸鼠不同组织中的表达及分析
- Author:
Jinwei YOU
;
Min DONG
;
Biao LIU
;
Lei LIANG
;
Ying TIAN
;
Wenjuan HU
;
Xiaoyun TIAN
;
Tian FANG
;
Senmei ZHOU
;
Zhigang ZHAO
;
Shifeng YUN
- Publication Type:Journal Article
- Keywords:
Green fluorescent protein ( GFP);
Nude mice;
Tissue distribution
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(5):67-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether the green fluorescent protein ( GFP) gene can be successfully expressed in green fluorescent nude mice and the tissue distribution characteristics.Methods Small animal imaging system and RT-PCR assay were used to detect the GFP tissue distribution and fluorescence expression level.Results The GFP can be expressed in multiple tissues in green fluorescent nude mice.A higher expression was observed in the pancreas, heart, brain, and skin.Conclusion Exogenous GFP can be stably expressed and inherited in green fluorescent nude mice, with the highest expression in the pancreas.
