Influences of fenofibrate on oxidative stress and endoplasmic reticulum stress in livers of hyperlipidemic rats
10.3969/j.issn.1006-5725.2014.17.008
- VernacularTitle:非诺贝特对高脂血症大鼠肝脏氧化应激和内质网应激的影响
- Author:
Weifeng ZHOU
;
Xinru SHEN
;
Lingyun WANG
;
Qiu ZHANG
;
Yunxia LU
- Publication Type:Journal Article
- Keywords:
Fenofibrate;
Hyperlipidemia;
Insulin resistance;
Oxidative stress;
Endoplasmic reticulum stress
- From:
The Journal of Practical Medicine
2014;(17):2718-2721
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influences of fenofibrate on oxidative stress and endoplasmic reticulum stress in livers of hyperlipidemic rats. Methods Male SD rats were fed with high-fat diet for 12 weeks to induce a model of hyperlipidemia, then divided into control group, high-fat diet group with another four-week high-fat diet and fenofibrate group, in which rats were treated with fenofibrate [100 mg / (kg·d)] for 4 weeks. Then improvement of insulin resistance was detected in rats with GTT and ITT experiment. The serum levels of glucose (GLU), fasting insulin (FINS),triglyceride (TG) and total cholesterol (TC) were detected. The pathological changes of livers were detected with Oil Red O staining. The oxidative stress indices such as T-SOD, Mn-SOD, GSH and T-AOC were detected with liver homogenate. The expression of GRP78 was detected with real-time quantification RT-PCR and Western blot analysis respectively. Results Compared with rats with high-fat-diet, rats after fenofibrate treatment showed obviously improved insulin resistance, lower serum level of TG, TC and FINS (P < 0.05), decreased number and size of lipid droplets in liver tissue sections. T-SOD level in liver homogenate was significantly increased (P < 0.05), while GSH and T-AOC levels increased but had no obvious differences when compared with control group (P > 0.05). The expression of GRP78 at mRNA and protein levels were increased significantly after fenofibrate treatment (P < 0.05). Conclusions Fenofibrate has significant effects on improving insulin resistance and lipid regulation, which might be related to decreased oxidative stress and subsequent endoplasmic reticulum stress.
