Effect of human bone marrow mesenchymal stem cells on proliferation of peripheral blood CD8 + T lymphocytes

VernacularTitle:骨髓间充质干细胞对外周血CD8＋T淋巴细胞增殖的体外实验研究
Author: Yan LIAO ; Mingfen LI ; Gang CHEN ; Xiaocong KUANG ; Haibin LI ; Dianzhong LUO
Publication Type:Journal Article
Keywords: Mesenchymal stem cells; Bone marrow; CD8+ T lymphocytes; Immune response
From: The Journal of Practical Medicine 2014;(17):2722-2725
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of human bone marrow mesenchymal stem cells (MSCs) on proliferation of CD8+ T lymphocytes and its mechanism. Methods MSCs were isolated and cultured then identified through many ways. The proliferative influence of MSCs on peripheral blood mononuclear cells (PBMCs) stimulated by PHA was investigated. The effect of MSCs on proliferation of CD8 + T lymphocytes induced by PHA was explored by flow cytometry. The possible mechanism of the inhibition effect of MSCs was investigated on the proliferation of CD8+ T cells stimulated by PHA with Transwell assay. Results MSCs were successfully harvested and cultured in vitro. MSCs suppressed the proliferation of CD8+ T cells stimulated by PHA when MSCs ∶ PBMCs ≥ 1 ∶ 5, which showed a dose-dependent manner. Strong proliferative inhibition of MSCs was presented on the CD8 + T cells induced by PHA in the group of Transwell (MSCs ∶ PBMCs = 1 ∶ 1) and the influence was similar to non-Transwell group. Conclusion MSCs can affect body immunity via suppressing the proliferation of CD8+ T cells.