Inhibition of LPS-induced expression of myeloid differentiation factor 88 by fenoterol is associated with its anti-inflammatory effect
10.3969/j.issn.1006-5725.2014.18.008
- VernacularTitle:非诺特罗对内毒素诱导的 MyD88表达发挥抑制作用与抗炎效应相关
- Author:
Wei WANG
;
Jie XU
;
Bei HE
- Publication Type:Journal Article
- Keywords:
Fenoterol;
Monocyte;
LPS;
Anti-inflammation;
Myeloid differentiation factor 88
- From:
The Journal of Practical Medicine
2014;(18):2890-2893
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism of inhibition of LPS-induced inflammation by fenoterol, a β2 adrenoceptor agonist in monocyte. Methods Concentrations of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α) and MCP-1 from cell supernatants from THP-1 cells and wild type or MyD88- / - mice peritoneal macrophages stimulated by LPS in the presence or absence of fenoterol were determined by use of an ELISA system. Expression of MyD88 (myeloid differentiation factor 88) stimulated by LPS in the presence or absence of fenoterol were determined by Western blot. Results Fenoterol inhibited LPS-induced activation of MyD88 and secretion of inflammatory cytokines (TNF-α, MCP-1, and IL-1β). The reaction of MyD88- / - mice peritoneal macrophages to LPS was much lower than that of the wild type mice peritoneal macrophages. Conclusions MyD88 plays an important role in inflammation induced by LPS. The inhibition of LPS-induced expression of MyD88 by fenoterol is associated with its anti-inflammatory effect.
