Effects of tetrandrine onβ-glucan induced RAW264 .7 cells proliferation
10.3969/j.issn.1673-4130.2014.19.001
- VernacularTitle:粉防己碱对β-葡聚糖诱导的 RAW264．7细胞增殖的作用
- Author:
Lanfang GUO
;
Xiaoqing CHENG
;
Huiyan NI
;
Xiaofen XU
;
Ping WEN
- Publication Type:Journal Article
- Keywords:
tetrandrine;
interleukin-6;
interleukin-10;
tumor necrosis factor-α;
prostaglandin E2
- From:
International Journal of Laboratory Medicine
2014;(19):2577-2578
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of tetrandrine on β-glucan induced RAW 264 .7 cells proliferation .Methods RAW 264 .7 cells model was established .Four methyl thiazol tetrazolium(MTT) was used to detect the effects of different concentrations of tetrandrine on the proliferation of RAW264 .7 cells .The levels of interleukin(IL)-6 ,tumor necrosis factor-α(TNF-α) ,prostaglan-din E2 (PGE2 ) and IL-10 in the culture supernatant were measured by enzyme linked immunosorbent assay(ELISA) .Results MTT results showed that the growth curves of different concentrations of tetrandrine on RAW264 .7 cells had biphasic affections .ELISA results suggested that tetrandrine could inhibit the IL-6 ,TNF-α,PGE2 expressions and promote IL-10 expression .Conclusion Effects of tetrandrine onβ-glucan induced RAW264 .7 cells proliferation are relative to inhibition of IL-6 ,TNF-α,PGE2 expressions and promotion of IL-10 expression .
