Effect of caspase-9 inhibitor on apoptosis in cartilage endplate chondrocytes in rat vertebrae
10.3969/j.issn.1005-4847.2014.05.004
- VernacularTitle:Caspase-9抑制剂对 SD 大鼠椎间盘软骨终板细胞凋亡的影响
- Author:
Wei LU
;
Defang LI
;
Bin ZHU
;
Jingping WU
- Publication Type:Journal Article
- Keywords:
Caspase-9 inhibitor;
Fetal bovine serum;
Cartilage endplate;
Apoptosis Chondrocytes;
Vertebra;
Rat
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(5):17-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of caspase-9 inhibitor on low fetal bovine serum ( FBS)-induced apop-tosis in cartilage endplate chondrocytes in SD rat vertebrae.Methods Disc cartilage endplates were obtained from 3-month old SD rats and subjected to sequential digestion to harvest chondrocytes for primary culture, and apoptosis was in-duced by 1%FBS for 48 hours.Three groups of chondrocytes were treated by 1% FBS, caspase-9 inhibitor ( Z-LEHD-FMK) and DMSO, respectively.After 48 hours, apoptosis was detected by DAPI staining and flow cytometry.The expres-sion of procaspase-9, active caspase-9 and active caspase-3 was monitored by Western blot.Results Compared with the 1%FBS group (40.8 ±0.84)%and DMSO group (40.2 ±1.56)%, the apoptosis rate of the caspase-9 inhibitor group (26.3 ±2.56)% was significantly lower (P<0.05).The expressions of active caspase-9 and active caspase-3 in the caspase-9 inhibitor group were significantly lower than those in the other two groups (P<0.05).Conclusions Caspase-9 inhibitor can inhibit low FBS-induced apoptosis in cartilage endplate chondrocytes of rat vertebrae, and might become a new drug for the treatment of disc degeneration.
