Cloning and analysis of tissue expression of PGC-1αgene in Guangxi Bama mini-pigs
10.3969/j.issn.1005-4847.2014.05.006
- VernacularTitle:广西巴马小型猪 PGC-1α基因克隆与组织表达分析
- Author:
Xueyu YAN
;
Qiuwei AO
;
Xiaodong YAN
;
Qingyang JIANG
;
Yafen GUO
;
Ganqiu LAN
- Publication Type:Journal Article
- Keywords:
Guangxi Bama mini-pig;
PGC-1αgene;
Sequencing analysis;
Tissue expression
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(5):27-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone the coding sequence of Guangxi Bama mini-pig PGC-1αgene, and to analyze the expression of PGC-1αgene in various tissues of mini-pigs using RT-PCR and QRT-PCR techniques.Methods The PGC-1αgene coding sequence ( CDS) was amplified by PCR from the cDNA of longissimus muscle of Guangxi Bama mini-pig. The PCR products were inserted into pEASY-T5 vector, transfected E.coli, identified and sequenced.The PGC-1αgene expression in different tissues of the Bama mini-pigs was detected by RT-PCR and QRT-PCR assays.Results The PGC-1αgene CDS of Guangxi Bama mini-pig was cloned.It was 2391 bp in length.It had 99.9%homology with the reference sequence, and had two synonymous mutations that were C-A1105 and G-A1524.The expression level of PGC-1αgene was higher in the heart and kidney, followed by liver, subcutaneous fat and longissimus muscle, but the expression was not de-tected in pancreas of Guangxi Bama mini-pig.Conclusions We have successfully cloned the PGC-1αgene of Guangxi Bama mini-pig, and detected this gene expression in six tissues.The results of this study will provide a basis for studying the effect of PGC-1αon type 2 diabetes mellitus (T2DM) in Bama mini-pigs.
