Establishment of a nude mouse model of pulmonary adenocarcinoma metastasis
10.3969/j.issn.1005-4847.2014.05.007
- VernacularTitle:肺癌转移瘤动物模型的建立
- Author:
Yusheng CHEN
;
Xiaofen LIN
;
Hongru LI
;
Xian LIN
;
Ming LIN
;
Nengluan XU
- Publication Type:Journal Article
- Keywords:
Lung adnocarcinoma;
Neoplasm metastasis;
Animal model;
Nude mice
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(5):32-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an optimal animal model of pulmonary metastasis of human lung adenocarcino-ma, to serve further investigation of mechanism of lung adenocarcinoma metastasis.Methods Eleven nude mice aged 4-6 weeks were used in this study.Suspension of human lung adnocarcinoma A549 cells (0.1 mL, 107 cells/mL) was injec-ted into the tail vein in nude mice.From four weeks after inoculation, two nude mice were killed each time at 4, 5, 6 weeks after the tumor cell injection at random for examination.The remaining 3 mice were killed at the end of the experi-ment.At autopsy, the lung, brain, liver, kidney and other organs were removed, fixed in neutral buffered formalin and embedded in paraffin.Sections were cut and stained with hematoxylin-eosin, and examined by histopathology.The number of metastatic foci was counted.Results No mouse died after tumor cell inoculation.Serially euthanized mice revealed evi-dence of gradually increasing pulmonary metastases in the mice:No metastasis was found before 4 weeks after tumor cell in-oculation, the first histological metastases appeared at 5 weeks, gross metastatic foci were observed at 6 weeks, widely spread metastatic foci were observed at 7 weeks, and the remain 3 mice developed cachesia at 11, 13, and 14 weeks after tumor cell inoculation.Mediastinal lymph node metastases were found in the nude mice by 11 weeks after tumor cell inocu-lation.Conclusions We have successfully established a nude mouse model of pulmonary metastasis by injecting human lung adenocarcinoma A549 cells into the tail vein.
