Development of loop mediated isothermal amplification for rapid detection of Escherichia coli
10.3969/j.issn.1673-4130.2014.19.042
- VernacularTitle:快速检测大肠埃希菌 LAM P方法的建立
- Author:
Chunyang REN
;
Tian TIAN
;
Jie TIAN
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
loop-mediated isothermal amplification;
rapid detection
- From:
International Journal of Laboratory Medicine
2014;(19):2668-2669
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply a one-step loop-mediated isothermal amplification(LAMP) assay for rapid detection of Escherichiaco-li .Methods According to the Genbank of Escherichiacoli lacZ(accession number:M74750) ,a set of four specific primers were designed for the Escherichiacoli LAMP assay and optimized the reaction .The results were judged with naked eyes and electrophoretic analysis .Results Genomic DNAs from 13 bacterial strains including Escherichia coli strains were amplified using LAMP ,and no amplicon was observed in the other bacterial strains .The detection limits of LAMP assay was 15 cfu/mL .Conclusion LAMP assay is extremely rapid ,cost-effective , specific and highly sensitive and has potential usefulness for clinical application .
