Effects of Jagged1 on hippocampal radial glial cells’ proliferation and neuronal differentiation
10.3969/j.issn.0529-1356.2014.05.001
- VernacularTitle:Jagged1对海马放射状胶质细胞增殖和向神经元分化的影响
- Author:
Jianbing QIN
;
Min CHENG
;
Guohua JIN
;
Haoming LI
;
Jinhong SHI
;
Linqing ZOU
;
Meiling TIAN
- Publication Type:Journal Article
- Keywords:
Radial glial cell;
Proliferation;
Differentiation;
Hippocampus;
Neurons;
Immunofluorescent;
Rat
- From:
Acta Anatomica Sinica
2014;(5):585-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Jagged1 on hippocampal radial glial cells (RGCs) proliferation and neuronal differentiation in vitro.Methods Hippocampal RGCs were cultured in vitro, the agonist Jagged1 and(or) inhibitor DAPT of Notch signaling were added into the culture medium , and then the cells were divided into control group , Jagged1 group, Jagged1 combined with DAPT group and DAPT group .CCK-8 regent was used to detect cells ’ vitality;immunofluorescent was used to detect the number of BLBP /Ki67 double labeled cells and differentiated microtubule associated protein-2(MAP-2) positive cells.Results Cell vitality in Jagged1 group was obviously higher than that of the other groups .The number of BLBP/Ki67 double labeled cells and differentiated MAP-2 positive cells were more than other groups.Conclusion Jagged1 promotes the proliferation and neuronal differentiation of hippocampal RGCs in vitro.
