Transplantation of stem cell-preseeded dentin slices incorporated with periodontal ligament cell sheets from beagle dog into nude mice
10.3969/j.issn.1001-3733.2014.05.02
- VernacularTitle:预培养比格犬干细胞的牙本质片复合牙周膜细胞膜片裸鼠体内移植研究
- Author:
Yuwang LU
;
Hongwei LIU
;
Xiaoyu WANG
- Publication Type:Journal Article
- Keywords:
Periodontal ligament cells(PDLCs);
Bone marrow stroma cells(BMSCs);
Cell sheet;
Periodontal regeneration;
Extracellular matrices(ECM)
- From:
Journal of Practical Stomatology
2014;(5):598-602
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of dentin proseeded with BMSCs or PDLCs incorporated with PDLCs sheets and calci-nated ceramic bovine bone(CBB)in the reconstruction of periodontal tissue.Methods:Root dentin slices were prepared and pre-seeded with BMSCs or PDLCs from beagle dog.After cultivated in osteoinduction medium(OST groups)orα-modified eagle's medi-um(MEM groups)for 3 weeks,the dentin slices observed by scanning electron microscope (SEM).Then the slices were wrapped successively with PDLCs sheets and CBB as new constructs(BMSCs or PDLCs/dentin/PDLCs sheets/CBB).The new constructs were transplanted into nude mice subcutaneously,8 week after transplantation,samples were havested and examined by HE staining.Re-sults:Enough cells and extracellular matrices were detected on the dentin slices by SEM in vitro.A little new immature cementum-like tissue without periodontal-like tissue on the surface of the new construcs was observed in OST groups.By contrast,in MEM groups,periodontal-like without immature cementum-like tissue formation was observed.Conclusion:Dentin proseeded with BMSCs or PDLCs incorporated with PDLCs sheets and CBB cultured by MEM can promote periodontal-like tissue regeneration.Cultured with osteooinduction medium can promote cementum-like tissue regeneration.
