The effects of rhPDGF-BB combined with rhTGF-β1 on FAK mRNA expression of osteoclasts in the alveo-lar bone on the pressure side of orthodontic teeth in rats
10.3969/j.issn.1001-3733.2014.05.07
- VernacularTitle:rhPDGF-BB与rhTGF-β1联合应用对大鼠正畸牙压力侧破骨细胞FAK mRNA表达的影响
- Author:
Jiangtao ZHANG
;
Mei MEI
;
Jianguo LIU
;
Ce JIANG
;
Lei FENG
;
Jin HUANG
- Publication Type:Journal Article
- Keywords:
rhPDGF-BB;
rhTGF-β1;
Orthodontic tooth;
Osteoclasts;
FAK
- From:
Journal of Practical Stomatology
2014;(5):619-623
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of recombinant human platelet-derived growth factor-BB(rhPDGF-BB)combined with recombinant human transforming growth factor-β1 (rhTGF-β1 )on the expression of FAK mRNA of osteoclasts in the alveolar bone on the pressure side of orthodontic teeth in rats.Methods:Orthodontic tooth movement model was established in 160 male SD rats.The rats in experimental group were treated by injection of 10 ng rhPDGF-BB and 5 ng rhTGF-β1 in the buccal submucosal area of the mo-lar every other day from day 1 afterburner,while those in control group received equivalent volumes of PBS.The rats were sacrificed at 1,4,7,10 and 14 days(n=16)after appliance placement.Specimens were collected.Osteoclasts in the alveolar bone on the pres-sure side of the orthodontic teeth were observed by TRAP staining,the FAK mRNA expression was quantified by quantitative RT-PCR.Results:rhPDGF-BB combined with rhTGF-β1 significantly promoted an increasing number of osteoclasts on the compressing side(P<0.05),increased the expression of FAK mRNA at day 4 and 7(P<0.05),then decresed it to the control level(P>0.05) at day 14.Conclusion:Combination of rhPDGF-BB and rhTGF-β1 can increase the number of osteoclasts in the alveolar bone on compressing side,and promote FAK mRNA expression in osteoclasts.
