The effects of Porphyromonas gingivalis infection on mononuclear cell adhesion to vascular endothelial cells

VernacularTitle:牙龈卟啉单胞菌感染对血管内皮细胞与单核细胞黏附的影响
Author: Hui DENG ; Yafei WU ; Yi DING
Publication Type:Journal Article
Keywords: Porphyromonas gingivalis; Vascular endothelial cells; Monocyte; Adhesion
From: Journal of Practical Stomatology 2014;(5):633-636
CountryChina
Language:Chinese
Abstract: Objective:To elucidate the effects of Pg in modulation of monocyte adhesion to human umbilical vein endothelial cells (HUVECs).Methods:The model of infection of HUVECs by Pg was established in vitro.HUVECs were infected with Pg381 and Pg33277 and then incubated with THP-1 monocytes respectively.The amount of THP-1 adhesion to HUVECs was tested by rose Ben-gal staining assay.Results:Pg381-infected HUVECs demonstrated significantly increased adhesion of THP-1 cells compared to those infected with Pg33277(0.210 ±0.025 vs 0.078 ±0.024,P<0.05)or control HUVECs(0.062 ±0.022,P<0.05)after 6 h cul-ture.There was no differences among the above three groups after 24 h culture(P>0.05).Conclusion:The infection of Pg381 can increased the adhesion of monocytes to HUVECs.