Detection and isolation of cancer stem cells in primary and metastatic carcinoma tissues of colorectal cancer

VernacularTitle:大肠原发癌和转移癌组织中肿瘤干细胞的检测与分选
Author: Ru JIA ; Qing SUN
Publication Type:Journal Article
Keywords: colorectal neoplasm; cancer stem cell; flow cytometry; sorting
From: Chinese Journal of Clinical and Experimental Pathology 2014;(9):1000-1003
CountryChina
Language:Chinese
Abstract: Purpose To detect and isolate cancer stem cells ( CSC) in fresh specimens of primary and metastatic carcinoma tissues from 10 cases of colorectal cancer to study the biological functions and mechanisms of migrating cancer stem cells. Methods Suspen-sions of primary and metastatic colorectal cancer cells were labeled with antibodies CD133-PE and CD44-FITC, and then flow cytometry was used to detect and isolate the CD133-PE-positive and CD44-FITC-positive cells. Results There were certain percentages of CD133-positive and CD44-positive cells present in primary and metastatic colorectal cancer and the proportion of CSC in metastatic colorectal cancer was higher than that in primary cancer tissue (P<0.05). Conclusions There are CSC in both primary and meta-static colorectal cancer and the proportion of CSC in metastatic colorectal cancer is significantly higher than that in primary cancer tis-sue, suggesting that CSC play an important role in the metastasis of colorectal cancer.