Effects of p38-2 G4 high-expression on the proliferation and erythriod differentiateon of murine erythroleukemia cells
10.3969/j.issn.0529-1356.2014.05.015
- VernacularTitle:p38-2 G4蛋白高表达对小鼠红白血病细胞增殖和分化的影响
- Author:
Xiaofang ZHU
;
Mingming SHI
;
Zuli YANG
;
Fukun ZHAO
;
Shifu ZHANG
- Publication Type:Journal Article
- Keywords:
p38-2G4;
Murine erythroleukemia;
Lentiviral transfection;
High-expression;
Western blotling;
Mouse
- From:
Acta Anatomica Sinica
2014;(5):670-674
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of mouse proliferation-associated protein 2G4 (p38-2G4) high-expression on the proliferation and erythriod differentiation of murine erythroleukemia ( MEL ) cells.Methods To establish the recombinant lentivirus vector p 38-2G4-pLJM1, the p38-2G4-pLJM1 was cotransfected into HEK293T cells to obtain lentivirus with pCMV-VSV-G and pCMV-dR8.2.Lentivirus were infected into MEL cells to establish the stably p 38-2G4 high-expressed MEL cells.Western blotting was used to analyse the high-expression efficiency.MTT assay and benzidine staining were applied to detect the cell viability and hemoglobin synthesis of the stable cell line in presence /absence of inducers.Results Western blotting showed that the p38-2G4 high-expression stable cell strain had a higher expression of p38-2G4 as compared to the control group ( MEL) ( P <0.05).MTT result showed that there was no difference between the p38-2G4 high-expression cell strain and the control group (P>0.05), but the hemoglobin synthesis had been reduced as compared to the control group (P<0.05).Conclusion p38-2G4 high-expression does not affect the cell viability of MEL cells , but inhibits the erythriod differentiation of MEL cells in three independent experiments .
