Expression of fibroblast growth factor-2 and fibroblast growth factor receptor-4 in human papillary thyroid carcinoma and their significances
10.3969/j.issn.0529-1356.2014.05.016
- VernacularTitle:成纤维细胞生长因子2、成纤维细胞生长因子受体4蛋白在人甲状腺乳头状癌中的表达及意义
- Author:
Qian LI
;
Li LI
;
Shuqin GUO
;
Yunliang ZHANG
;
Fanqiang KONG
;
Fei LI
;
Junpeng KANG
;
Jingfang WU
;
Fulu GAO
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor-2;
Fibroblast growth factor receptor-4;
Invasion;
Metastasis;
Papillary thyroid carcinoma;
Western blotting;
Human
- From:
Acta Anatomica Sinica
2014;(5):675-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of fibroblast growth factor-2 (FGF-2) and fibroblast growth factor receptor-4 ( FGFR-4 ) in the papillary thyroid carcinomas ( PTC ) and clinical significance . Methods Immunohistochemistry and Western blotting for the expression of FGF-2 and FGFR-4 were performed in 89 cases of PTC and 30 cases of normal thyroid tissues ( NTT) adjacent to the tumors .Results Immunohistochemistry results showed that , FGF-2 and FGFR-4 expressions were high in thyroid carcinoma (P<0.01,P<0.01) in contrast to that in the normal thyroid tissues, and the difference was statistically significant;There was a positive linear correlation between expressions of FGF-2 and FGFR-4 and lymph node metastasis (χ2 =14.798,P<0.01;χ2 =7.27,P<0.01)and differentiation degree (χ2=13.824,P<0.01;χ2 =16.921, P<0.01) in papillary thyroid carcinoma ,while there was no difference in gender ,age and tumor size(P>0.05).Analyzed by Western blotting technique ,FGF-2 and FGFR-4 expressions in thyroid carcinoma were significantly higher than that in normal tissue ,with decrease of cancer degree of tissue differentiation and significantly up regulated expression (P<0.05).Expressions of FGF-2 and FGFR-4 were in a positive linear correlation in the disease (rs=0.434,P<0.01).Conclusion The expressions of FGF-2 and FGFR-4 are correlated with papillary thyroid cancer and they participated in the process of invasion and metastasis , both of which have a positive synergistic effect .The degree of malignancy and biological behavior are meaningful and comprehensive indicators ,which provide a theoretical basis for the subsequent experimental studies of cellular and molecular biology .
