Isolation,cultivation and differential proteomic analysis of chondrocytes from TMJ condylar cartilage of the mechanical stress stimulated rats
10.3969/j.issn.1001-3733.2014.05.01
- VernacularTitle:体内压应力刺激下大鼠髁突软骨细胞的分离、体外培养以及差异蛋白质组学观察
- Author:
Huang LI
;
Tuojiang WU
;
Xiangyu ZHANG
;
Qiaoling MA
;
Zichun HUA
- Publication Type:Journal Article
- Keywords:
Chondrocyte;
Mechanical stress;
Cell culture;
Rat;
Proteomics
- From:
Journal of Practical Stomatology
2014;(5):593-597
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the biological characteristics of condylar chondrocytes isolated from mechanical stress stimulated TMJ condylar cartilage of rats.Methods:The rat models of mechanical stress stimulated condylar cartilage were made by class III orthope-dic force for 14 days.The cartilage from control and experimental rats were observed in gross view.The cell harvest rate and viability were examined,proteomic analysis was performed.Results:After stress stimulation the thickness,the elasticity of condylar cartilage and number of chondrocytes were significantly reduced.The weight of mandibular cartilage was decreased(P<0.01).The harvest rate and the viability rate of the cells were decreased(P<0.01).The chondrocytes in the experimental group appeared to be elongated. Proteomic analysis showed that stress-related proteins,signal transduction proteins were up-regulated;cytoskeleton proteins,cell prolif-erative proteins were down-regulated.Conclusion:Mechanical stress stimulation of condylar cartilage may result in biological activity and protein changes of the condylar cartilage chondrocytes.
