The effects of sustained-release coating of HU-308 on the biological characteristics of rat osteoblasts

VernacularTitle:HU-308缓释涂层对大鼠成骨细胞生物学特性的影响
Author: Xueying ZHU ; Qian XU ; Qing FENG ; Rong XIANG
Publication Type:Journal Article
Keywords: HU-308; Titanium; Osteoblasts; Layer-by-layer assembly(LBL)
From: Journal of Practical Stomatology 2014;(5):607-610
CountryChina
Language:Chinese
Abstract: Objective:To observe the the effects of sustained-release coatings of HU-308 on the biological characteristics of rat oste-oblasts(ROBs)on Ti surface.Methods:The heparin/chitosan sustained release coatings containing HU-308 at different concentra-tions were prepared by layer-by-layer assembly technology on the alkali-and heat-treated Ti surface.The alkali-and heat-treated Ti samples were randomly divided into six groups,the samples in C group were used as control,in P group were treated by heparin/chi-tosan coatings and in T1,T2,T3 and T4 groups were treated by heparin/chitosan sustained-release coating containing HU-308 at dif-ferent concentrations.The adhesion,proliferation and ALP activity of ROBs cultured on the coatings were assessed at different times. Results:The adhesion,proliferation and ALP activity of ROBs of T4 group (the impregnating concentration of HU-308 was 0.025 mg/L)were the highest.Conclusion:The sustained-release coating with low concentration of HU-308 may improve the adhesion , proliferation and ALP activity of ROBs.