The effects of sulforaphane on the proliferation and apoptosis of salivary adenoid cystic carcinoma ACC-M cells
10.3969/j.issn.1001-3733.2014.05.015
- VernacularTitle:莱菔硫烷对涎腺腺样囊性癌细胞系ACC-M增殖和凋亡的影响
- Author:
Zhiyu JIA
;
Zhizheng ZHUANG
;
Lei YUE
;
Tao GUO
;
Wei YANG
;
Yinghuai ZHANG
- Publication Type:Journal Article
- Keywords:
Sulforaphane(SFN);
Salivary adenoid cystic carcinoma;
Cell culture;
Proliferation;
Apoptosis
- From:
Journal of Practical Stomatology
2014;(5):653-657
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of sulforaphane(SFN)on the proliferation and apoptosis of salivary adenoid cystic carci-noma ACC-Mcells in vitro.Methods:ACC-Mcells were treated with SFN at the doses(μmol/L)of 5,10,20,30,40,60,80 and 100 for 24,48 and 72 hours,respectively.The growth inhibition was examined with MTT assay and typan blue exclusion assay.Morpholo-gy of ACC-M cells was observed with phase contrast microscope,giemsa staining and transmission electron microscope.Flow cytome-try with Annexin-V-FITC/PI double staining was used to detect the apoptosis of ACC-M cells.Results:SFN inhibited the prolifera-tion of ACC-Mcells,the IC50 values(μmol/L)after 24,48 and 72 h treatment were 75.6,21.3 and 16.5 respectively.The highest inhibition rate was 89.2%.The growth inhibition rate of SFN on ACC-Mcells was positively correlated with concentrations of SFN and treatment time.SFN induced the apoptosis of ACC-Mcells in a dose and time dependent manner(P<0.01).Conclusion:SFN can inhibit proliferation and induce apoptosis of salivary adenoid cystic carcinoma ACC-M cells time and dose-dependently.
