Expression and correlation of PKCζ, MMP-2 and MMP-9 in breast cancer
10.13315/j.cnki.cjcep.2014.09.003
- VernacularTitle:乳腺癌中PKCζ、MMP-2、MMP-9的表达及相关性
- Author:
Shuo YANG
;
Hongli LI
;
Wentong LI
;
Lu YANG
;
Ming NI
;
Limin ZHAI
;
Chonggao YIN
;
Baogang ZHANG
- Publication Type:Journal Article
- Keywords:
breast neoplasm;
PKCζ;
MMP-2;
MMP-9;
invasion;
metastasis
- From:
Chinese Journal of Clinical and Experimental Pathology
2014;(9):958-962
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expressions of PKCζ, MMP-2, and MMP-9 in breast cancer and the relationship with the inva-sion and metastasis of breast cancer. Methods The expression of PKCζ, MMP-2 and MMP-9 in 100 cases with breast cancer was as-sessed with immunohistochemistry PV 9000 method. PKCζ-siRNA was transfected into MDA-MB-231 cell lines, called siPKCζ/MDA-MB-231. While siRNA construct containing a scrambled sequence was transfected into MDA-MB-231 cells to generate control cells, which were designated as Scr/MDA231 cells. Western blotting was used to measure the expression of PKCζ in transfected cells, and the Transwell invasion assay was used to detect the invasion ability in vitro. The content of MMP-2, MMP-9 were measured by ELISA. Results The expression rates of PKCζ, MMP-2 and MMP-9 in breast cancer tissues were 62.5%, 37.5% and 32.5%, and there were significant differences among them (P<0.05). The expression of PKCζwas much higher than those in the normal breast tissues nearby. The expression of PKC protein was assoiated with lymph node metastasis, distant metastasis (P<0.01), but was not correla-ted with other clinicopathologic parameters, such as age, tumor size, histological type, ER, PR, and so on (P>0.05). The expres-sion of PKCζ, MMP-2 and MMP-9 were lower in siPKCζ/ MDA-MB-231 group than those in scr/ MDA-MB-231 group, and the in vitro invasion ability was significantly decreased (P<0.05). Conclusions PKCζ can promote the invasion and metastasis of breast canc-er, and correlated with the expression of MMP-2, MMP-9(P<0.05).
