Determination of Bergeni in Herba Ardisiae Japonicae by Capilary Zone Electrophoresis
- VernacularTitle:毛细管电泳法测定矮地茶中岩白菜素的含量
- Author:
Wenting KONG
;
Liyu PAN
;
Baiyun ZHAO
- Publication Type:Journal Article
- Keywords:
Herba Ardisiae Japonicae;
Bergeni;
Capilary zone electrophoresis;
Determination
- From:
China Pharmacist
2014;(12):2151-2153
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of bergeni in Herba Ardisiae Japonicae. Methods: Herba Ardisiae Japonicae was ultrasonic extracted by methanol after crushed and sieved. An untreated fused-silica capillary (75 μm × 48. 5 cm, 40 cm effective length) was used, the running buffer was 25 mmol·L-1 borax in water, the voltage was 15 kV, the temperature was 25℃, the internal standard was cinnamic acid, the ultraviolet wavelength was set at 240nm, and the running time was 10 minute. Results:Bergeni, the internal standard and the impurities were separated by baseline under the above conditions. Bergeni was linear within the range of 3. 356-167. 800μg·ml-1 with the linear equation of Y =0. 034 9X+0. 060 6(r =0. 997 9), the limit of determination was 0. 839 μg·ml-1(S/N = 3), the limit of quantitation was 3. 356 μg·ml-1(S/N = 10), the average recovery was 99. 6%(RSD = 3. 1%, n = 6), and RSDs of intra and inter-day were lower than 5% (n = 3). Conclusion: The method has the advantages of convenience, fastness, accuracy, and low sample and reagent consumption, which can be used to determine bergeni in Herba Ardisiae Japonicae.
