Comparative Study on Concentration Monitoring of CsA in Human Whole Blood by EMIT and HPLC
- VernacularTitle:EMIT法与HPLC法测定人全血环孢素谷浓度的比较
- Author:
Xia XU
;
Songgang JI
;
Xiangyu HOU
;
Ping LENG
;
Chunyan WANG
- Publication Type:Journal Article
- Keywords:
Enzyme multiplied immunoassay test;
HPLC;
CsA;
Blood concentration
- From:
China Pharmacist
2014;(12):2003-2006
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the difference and correlation of HPLC and enzyme-multiplied immunoassay test( EMIT) for the determination of CsA in human whole blood. Methods:A total of 119 clinical samples at different concentrations of CsA were collected and respectively determined by HPLC and EMIT. The difference and correlation of the two determination methods were investigated. Results:There was significant difference in the blood concentrations of CsA determined by HPLC and EMIT(P<0. 05). CsA concen-tration determined by EMIT was 26. 2 ng·ml-1 higher than that determined by HPLC, and 95% CI was (14. 6-37. 7) ng·ml-1 . A satisfactory correlation was achieved between the two methods(r=0. 997 4). Conclusion:There is statistically significant difference in the CsA concentration in whole blood respectively determined by EMIT and HPLC. Attention should be paid to CsA monitoring by E-MIT and HPLC, and relevant adjustment should be carried out.
