Relationship between carotid artery intima-media thickness and apolipoprotein E and angiotensin converting enzyme gene polymorphism in patients with diabetes mellitus.
- Author:
Won KIM
1
;
Jung Pil YEOM
;
Dal Sik KIM
;
Tae Sun PARK
;
Hong Sun BAEK
;
Sung Kyew KANG
;
Sung Kwang PARK
Author Information
1. Department of Internal Medicine, Chonbuk National University Medical School, Chonju, Korea.
- Publication Type:Original Article
- Keywords:
Diabetes mellitus;
Apolipoproteins E;
polymorphism;
Peptidyl-dipeptidase A;
Carotid arteries
- MeSH:
Albuminuria;
Angiotensins*;
Apolipoproteins E;
Apolipoproteins*;
Carotid Arteries*;
Carotid Artery, Common;
Carotid Intima-Media Thickness;
Diabetes Mellitus*;
Gene Frequency;
Genotype;
Humans;
Logistic Models;
Male;
Multiplex Polymerase Chain Reaction;
Peptidyl-Dipeptidase A*;
Polymerase Chain Reaction;
Ultrasonography
- From:Korean Journal of Medicine
2000;58(6):639-650
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: To study the distribution of the polymorphism of apo E and angiotensin converting enzyme genotypes in type 2 diabetic patients and to evaluate possible association between the apo E genotypes and angiotensin converting enzyme genotypes and intima-media thickness of the common carotid artery. METHODS: Study participants were 206 type 2 diabetic patients (112 men and 94 women), aged 18-81 years. HbA1C, albuminuria, and lipid status were assessed by standard laboratory techniques ; the apo E genotypes were assessed by modified amplification refractory mutation system of polymerase chain reaction technique and the angiotensin converting enzyme genotypes were assessed by multiplex polymerase chain reaction technique. The intima-media thickness was measured by high-resolution ultrasonography. RESULTS: The apo E allele frequencies of patients were E2 11%, E3 72%, and E4 17%. Mean HDL-cholesterol was lower in E4 carrier (n=35) than E2 (n=148) and E3 (n=35) carrier. E2 carriers has less common carotid intima-media thickness than E3 and E4 carriers (p< 0.05). The angiotensin converting enzyme genotypes were distributed as follows ; II 38%, ID 47%, DD 15%. The intima-media thickness value did not differ among patients with various genotypes. Multiple logistic regression analysis showed that only apo E polymorphism was determinant for the intima-media thickness. CONCLUSION: Our results suggested that apo E polymorphism was associated with carotid artery intima-media thickness in type 2 diabetic patients. But we could not find an association between carotid artery intima-media thickness and angiotensin converting enzyme genotype in this patients population.
