Determination of Two Components and Preservative in Compound Dextromethorphan Hydrobromide Syr-ups by HPLC
- VernacularTitle:HPLC法测定复方氢溴酸右美沙芬糖浆中2组分及防腐剂的含量
- Author:
Xinghan HUANG
;
Luhua FEI
- Publication Type:Journal Article
- Keywords:
Compound dextromethorphan hydrobromide syrups;
Dextromethorphan hydrobromide;
Guaifenesin;
Benzoic acid;
De-termination;
HPLC
- From:
China Pharmacist
2014;(12):2056-2058
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of two components and the preservative in compound dextromethorphan hydrobromide syrups. Methods:An Agilent Zobax SB-C18 column(250 mm × 4. 6 mm,5 μm) was used with meth-anesulfonic acid solution (adding 4. 8g methanesulfonic acid and 10ml triethylamine into 750ml water,and adjusting the pH value to 3. 5 by phosphoric acid)-acetonitrile (75∶25) as the mobile phase at the flow rate of 1. 0 ml·min-1 and 280nm as the detection wave-length. Results:The calibration curve was linear within the range of 102-1 025μg·ml-1 for guaifenesin,15-619μg ·ml-1 for dextro-methorphan hydrobromide and 10-407μg·ml-1 for benzoic acid. The average recovery of guaifenesin, dextromethorphan hydrobromide and benzoic acid was 100. 0% (RSD=0. 35%), 100. 1%(RSD=0. 77%)and 100. 8%(RSD=0. 49%), respectively. Conclusion:The method is simple,rapid and accurate,and suitable for the quality assessment of compound dextromethorphan hydrobromide syrups.
