Inhibition of Tau phosphorylation protects SH-SY5Y cells from MPP +-induced apoptosis by 6-Hydroxy-1H-indazole
10.3969/j.issn.1001-1978.2014.12.008
- VernacularTitle:6-羟基-1H-吲唑抑制 Tau 磷酸化对 MPP ＋诱导凋亡的SH-SY5Y 细胞保护作用的研究
- Author:
Wenting ZHU
;
Xiaofeng LIANG
;
Jinjun RAO
;
Wenya WANG
- Publication Type:Journal Article
- Keywords:
6-Hydroxy-1 H-indazole;
Tau;
MPP +;
SH-SY5Y;
apoptosis;
Parkinson’s disease
- From:
Chinese Pharmacological Bulletin
2014;(12):1655-1660
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the protective effect of 6-Hydroxy-1 H-indazole via inhibition of Tau phosphoryla-tion on MPP +-induced SH-SY5Y cells apoptotsis. Methods An apoptotic model induced by 1 -methy-4-phenylpridnium ion (MPP +)was established in cul-tured human neuroblastoma SH-SY5Y in vitro.MTT a-nalysis was used to evaluate the protective effect of pre-treatment of cells with 6-Hydroxy-1 H-indazole for 2 h. Hoechst33258 staining was used to observe apoptotic situation.Immunohistochemistry was used to detect the p-Tau (Ser396)expression.Results The viability of cells exposed to 200 μmol · L -1 MPP + for 48h was (47.80 ±0.84)% (P <0.01 ),MPP + induced phos-phorylation of Tau at Ser396 and neuronal apoptosis, while 6-Hydroxy-1 H-indazole inhibited MPP +-induced cellular apoptosis,increased the number of TH-positive cells via inhibiting Tau phosphorylation.Conclusion These results indicate that Tau may be a new target used to cure neurodegenerative disorders such as PD.
