Borneol activated volume-sensitive chloride channels in poorly differentiated nasopharyngeal carcinoma cells
10.3969/j.issn.1001-1978.2014.12.011
- VernacularTitle:冰片对鼻咽癌细胞容积敏感性氯通道的激活作用
- Author:
Long MENG
;
Haibo WANG
;
Zhiqin DENG
;
Yuan WANG
;
Jiabao WU
;
Zhouyi LAI
;
Ruiling LV
;
Xiaoxue SUN
;
Linyan ZHU
;
Lixin CHEN
;
Liwei WANG
- Publication Type:Journal Article
- Keywords:
Borneol;
chloride channels;
chloride channel blocker;
tumor cells;
patch clamp technique;
cell volume
- From:
Chinese Pharmacological Bulletin
2014;(12):1671-1676
- CountryChina
- Language:Chinese
-
Abstract:
Aim To clarify the effect of Borneol on the chloride channels and cell volume in poorly differentia-ted nasopharyngeal carcinoma CNE-2Z cells.Methods The technique of whole-cell patch clamp was used to detect the chloride currents and analyze the character-istics of the currents in CNE-2Z cells.The volume changes caused by Borneol were measured by the meth-od of time-lapse live cell imaging.Results The chlo-ride currents were induced by extracellular application of Borneol (20 μmol·L -1 )isotonic condition.The currents showed a characteristic of outward rectification and did not show voltage-dependent or time-dependent inactivation.The reversal potential of the currents was close to the CI-equilibrium potential. The currents were inhibited by the chloride channel blocker tamox-ifen.The currents were also inhibited by 47% hyper-tonic solution.Borneol decreased the cell volume by 9.4% in 30 min.Tamoxifen completely inhibited the Borneol-induced cell volume decrease.Conclusion Borneol can activate volume-sensitive chloride channels and induce volume decrease in CNE-2Z cells.Chloride channels play a pivotal role in the process of volume decrease caused by Borneol.
