Construction and activity characterization of E-cadherin and N-cadherin promoters
10.7644/j.issn.1674-9960.2014.11.005
- VernacularTitle:E-钙黏蛋白和N-钙黏蛋白启动子的构建及活性测定
- Author:
Lei ZHOU
;
Yanan ZHANG
;
Jie LIU
;
Lihua DING
;
Qinong YE
- Publication Type:Journal Article
- Keywords:
E-cadherin;
N-cadherin;
promoter;
luciferase reporter gene
- From:
Military Medical Sciences
2014;(11):860-862
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone E-cadherin and N-cadherin promoters and insert them into a luciferase reporter gene vector, and to characterize the promoter activity of E-cadherin and N-cadherin.Methods E-cadherin and N-cadherin pro-moter were cloned into pGL 4-basic.The resulting plasmids were determined by DNA sequencing .The promoter activity was analyzed in breast cancer cell line ZR 75-1 and hepatocarcinoma cell line HepG 2.Results DNA sequencing showed that the sequences of the cloned promoter regions were correct .Analysis of the reporter gene activity indicated that the E-cad-herin and N-cadherin promoters had the highest transcriptional activity in ZR 75-1 and HepG2 cells.Conclusion The E-cadherin and N-cadherin promoter genes are cloned successfully , contributing much to screening transcription factors that regulate E-cadherin and N-cadherin expression .
