Effect of BNIP3-induced autophagy in gastric carcinoma
10.3969/j.issn.1001-1978.2014.12.022
- VernacularTitle:BNIP3影响自噬水平对胃癌的影响
- Author:
Jiefei MIAO
;
Feng WU
- Publication Type:Journal Article
- Keywords:
BNIP3;
autophagy;
gastric carcinoma;
CoCl2;
hypoxia;
LC3-Ⅱ
- From:
Chinese Pharmacological Bulletin
2014;(12):1730-1733
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of BNIP3 on hy-poxia-induced autophagic cell death in gastric carcino-ma.Methods The protein levels of BNIP3 and LC3-Ⅱ of 1 8 cases of gastric carcinoma were studied with immunocytochemistry and Western blot analysis be-tween normal and tumor tissues.The expression of LC3-Ⅱ in SGC-7901 cells was assessed while knock-down of BNIP3 by siRNA in CoCl2-induced hypoxia, and the effect of 3-MA was also studied.Cell viability in hypoxia treatment for 48 h was estimated with cell counting kit-8 (CCK8).Results Compared with nor-mal tissues,the protein levels of BNIP3 and LC3-Ⅱwere increased in tumor tissues.BNIP3 siRNA could decrease the expression of LC3-Ⅱ and enhance the cell viability,while inhibition of autophagy could also in-crease the cell viability in SGC-7901 cells.Conclu-sions BNIP3 may play a role in hypoxia-induced cell death in gastric carcinoma,and the possible mecha-nism may be related to the regulation of autophagy.
