Role of ERO1αand its DNA methylation in homocysteine-induced inhibition of hepatocyte proliferation
10.3969/j.issn.1001-1978.2014.12.025
- VernacularTitle:ERO1α及其 DNA 甲基化在同型半胱氨酸抑制肝细胞增殖中的作用
- Author:
Li ZHAO
;
Chengjian CAO
;
Xianmei LIU
;
Fanqi KONG
;
Wenbin MA
;
Longxia ZHOU
;
Jiukai CHEN
;
Minghao ZHANG
;
Yun JIAO
;
Xiaoling YANG
;
Yideng JIANG
- Publication Type:Journal Article
- Keywords:
homocysteine;
ERO1 α;
hepatocytes;
proliferation;
poomoter;
DNA methylation
- From:
Chinese Pharmacological Bulletin
2014;(12):1743-1747
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the role of ERO1 αand its DNA methylation in homocysteine (Hcy)-induced in-hibition of hepatocytes proliferation.Methods The hepatocytes stimulated with 0 μmol·L -1 Hcy were set as the normal group (NC group)and the hepatocytes stimulated with 1 00 μmol·L -1 Hcy as the experimen-tal group (Hcy group).Methyl thiazolyl tetrazolium (MTT)reduction assay was used to reflect the prolifer-ation of the hepatocytes;qRT-PCR and Western blot were used to detect the mRNA and protein levels of ERO1 α;the expression of green fluorescence protein was observed in hepatocytes after the recombinant plas-mid of ERO1 α was constructed,which was used to confirm if the recombinant plasmid into hepatocytes was successful,then the mRNA and protein levels of ERO1 αwere assayed and the proliferation of the hepa-tocytes was also detected;ntMSP was used to detect the change of ERO1 αDNA methylation.Results The mRNA and protein levels of ERO1 αwere decreased in Hcy group compared with NC group,and the prolifera-tion activity of hepatocytes in Hcy group was de-creased.Sequencing result showed that the recombi-nant plasmid of ERO1 αwas constructed successfully. QRT-PCR and Western blot revealed that ERO1 αwas overexpressed. The result of MTT suggested that ERO1 αoverexpression restored hepatocyte proliferation inhibited by Hcy.Hcy caused ERO1 αDNA hyperm-ethylation.Conclusions Hcy inhibits hepatocyte pro-liferation by downregulating the expression of ERO1 α, and methylation of ERO1 αpromoter may play a role in this process.
