Effects of acteoside on expression of caspase-3 in cerebral cortex of mouse models of Alzheimer’s disease
10.3969/j.issn.1001-1978.2014.12.029
- VernacularTitle:类叶升麻苷对阿尔采末病小鼠皮层 caspase-3基因表达的影响
- Author:
Xiaoming PENG
;
Li GAO
;
Shixia HUO
;
Ming YAN
- Publication Type:Journal Article
- Keywords:
acteoside;
Alzheimer′s disease;
D-galac-tose;
AlCl3;
mice;
cerebral cortex;
caspase-3;
expres-sion
- From:
Chinese Pharmacological Bulletin
2014;(12):1763-1768
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of acteoside (AS)on the expression of caspase-3 in cerebral cortex of mouse models of Alzheimer’s disease(AD).Meth-ods Kunming (KM)strain mice were assigned into control group,model group,positive control group (VitE)and acteoside group.Every group was induced by a combination of D-galactose(i.p.60mg·kg -1 · d -1 )and AlCl3 (i.g.5mg·kg -1 ·d -1 )for 60ds ex-cept for control group,then mice were treated by dif-ferent concentrations(30,60,1 20 mg·kg -1 ·d -1 )of acteoside for 30ds.During the time,mice were in-duced continuously by a combination of D-galactose and AlCl3 .The learning and memory of mice were de-tected by step-down test,the activity of AChE in serum and brain of mice was measured by chemical colorime-try,the structure changes in cerebral cortex were ob-served by HE staining,and the expression of caspase-3 in cerebral cortex was analyzed through the immunohis-tochemical staining.Results Compared with model group,acteoside could improve the learning and mem-ory abilities(P <0.05 or P <0.01 ),decrease the ac-tivity of AChE in serum and brain(P <0.05 or P <0.01 ),and improve the morphology and number of neuron in cerebral cortex(P <0.01 ).Moreover,acte-oside could significantly inhibit the expression of caspase-3 in cerebral cortex (P <0.05,P <0.01 ). Conclusion Acteoside has significantly protective effects on brain damage of mice induced by a combina-tion of D-galactose and AlCl3 , and it′s protective mechanism probably relate to inhibiting the expression of caspase-3 and maintainings the normal morphology and number of neuron in cerebral cortex.
