Rapid detection of aflatoxin M1 in milk powder and milk based on up-converting phosphor technology
10.7644/j.issn.1674-9960.2014.11.003
- VernacularTitle:基于上转发光技术的奶粉及牛奶中黄曲霉毒素M1快速定量检测方法研究
- Author:
Xiao LIU
;
Liping WANG
;
Lei ZHOU
;
Chengxi LIN
;
Ruifu YANG
;
Jingfu QIU
- Publication Type:Journal Article
- Keywords:
up-converting phosphor technology;
lateral flow assay;
aflatoxin M1;
rapid detection;
quantitative detec-tion;
dairy product
- From:
Military Medical Sciences
2014;(11):850-854
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop and evaluate an up-converting phosphor technology-based lateral flow assay ( UPT-LF) for detection of aflatoxin M1(AFM1) in milk powder and milk.Methods AFM1-UPT-LF was established with up-converting phosphor ( UCP) nano-particles as the bio-label of competitive mode based LF assay .Sensitivity, quantitative ability and precision were evaluated using simulated AFM 1-postive samples with serial standard concentrations .The qualita-tive and quantitative detection performance of AFM 1-UPT-LF was evaluated with reference to liquid chromatography-mass spectrography ( LC-MS) to detect samples of milk powder and milk simultaneously .Results AFM1-UPT-LF could conduct qualitative and quantitative detection without sample pretreatment within 20 min.The detection limit of AFM1-UPT-LF reached 0.1 μg/kg in milk powder and 0.3 μg/L in milk.There was good linearity ranging from 0.1 to 0.7 μg/kg and 0.3 to 0.7 μg/L for milk powder and milk, respectively.The sensitivity, specificity and receiver operating characteristic ( ROC) area under the curve ( AUC) of AFM1-UPT-LF for qualitative result could meet the need of national standards for AFM1 limit in dairy products.After statistical analysis, there was no significant difference (milk powder: t=0.66, P>0.05;milk:t=1.01, P>0.05) between AFM1-UPT-LF and LC-MS for quantitative detection .Conclusion The good qualitative and quantitative detection performance of AFM 1-UPT-LF for milk powder and milk makes possible on-site rapid detection of AFM1 in dairy products quantitatively .
