Effect of lentiviral vector-mediated GDNF up-regulation on neuropathic pain of chronic constriction injury rats
10.3760/cma.j.issn.1008-1372.2014.12.002
- VernacularTitle:慢病毒介导GDNF过表达对CCI大鼠神经病理性疼痛的影响
- Author:
Zhuofeng DING
;
Wei XU
;
Zongbin SONG
;
Wangyuan ZOU
;
Qulian GUO
- Publication Type:Journal Article
- Keywords:
Lentivirus/genetics;
Genetic vectors;
Nerve growth factors;
Sciatic nerve/injuries;
Chronic disease;
Neuralgia/etiology;
Rats,Sprague-Dawley
- From:
Journal of Chinese Physician
2014;16(12):1590-1592
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of intrathecal injection of lentiviral vector-mediated up-regulation of glial cell line-derived neurotrophicfactor (GDNF) on neuropathic pain of chronic constriction injury (CCI) rats.Methods The CCI model was prepared by ligating the sciatic nerve of Sprague-Dawley (SD) rats.Seven days after CCI modeling,a single intrathecal injection of lentiviral vectors (LV)-GDNF was given.Before CCI and 3,5,7,14,and 21 days after CCI modeling,the mechanical pain threshold was tested in rats,and 21 days after surgery,Western blot was used to detect the expression of GDNF protein.Results On 21 days after CCI modeling,GDNF expression was reduced compared to sham group.After intrathecal injection of LV-GDNF,GDNF expression was up-regulated in the spinal cord,and CCI-induced mechanical hyperalgesia in rats was alleviated.Conclusions Intrathecal injection LV-GDNF can up-regulate the expression of GDNF and alleviate neuropathic pain in CCI rats.
