Rizatriptan effects on the cholecystokinin expression in endogenous pain modulatory system of a rat mi-graine model
10.3760/cma.j.issn.1674-6554.2014.11.001
- VernacularTitle:利扎曲普坦对偏头痛大鼠内源性痛觉调制系统缩胆囊肽表达的影响
- Author:
Gang YAO
;
Tingting HAO
;
Qian HUANG
;
Tingmin YU
- Publication Type:Journal Article
- Keywords:
Rizatriptan;
Migraine;
Periaqueductal gray;
Cholecystokinin
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2014;(11):961-963
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the influence of Rizatriptan on the cholecystokinin( CCK) expression in periaqueductal gray( PAG) of migraine model rat to investigate the possible mechanism by which Triptans treat mi?graine. Methods A total of 24 rats were randomly divided into four groups:normal control groups(A),migraine model groups(B),Rizatriptan control groups(C) and Rizatriptan treatment groups(D).C and D groups were intra?gastrically perfused with Rizatriptan,1 mg/kg per day. After 7 days,nitroglycerin was subcutaneously injected into the buttocks of the B and D group to induce migraine. Two hours after nitroglycerin injection,the trigeminal ganglia were isolated.CGRP expression in periaqueductal gray were determined using SYBR Green I real?time quantitative PCR and Immunohistochemistry. Results CCK mRNA levels ( target gene mRNA copies per 250 ng total RNA,× 106) in the rat midbrain of A,B,C,D groups were 1.25±0.41,1.71±0.93,0.17±0.12,0.22±0.07 respectively. CCK?8?immunoreactive positive cells in the rat PAG of each group were 37.17±12.62,40.17±11.09,27.33±7.71, 20.67±7.66 respectively. CCK mRNA expression in group C was significantly lower than that of group A(P<0.05) while the CCK mRNA expression in group D was lower than that of group B(P<0.05).The CCK?8?positive cells of the rat PAG in group D were lower than that in group B(P<0.05) . Conclusion Rizatriptan can down regulate the expression of CCK?8 in the PAG of the migraine rats and weaken the CCK?8 induced inhibition of the analgesic effects of opioid peptides.
