Effects of Monocrotaline on Right Ventricular Function and Expression of Cardiac Canonical Transient Receptor Potential Channels Subfamily in Experimental Rats
10.3969/j.issn.1000-3614.2014.11.019
- VernacularTitle:野百合碱预处理对大鼠右心室功能及规范性瞬时感受器电位亚家族表达的影响
- Author:
Kefeng CAI
;
Huiqin CHEN
;
Xunfa XU
;
Weiqiang LIN
;
Chaoxiang XU
;
Guozhen CHEN
- Publication Type:Journal Article
- Keywords:
Monocrotaline;
Canonical transient receptor potential channels;
Cardiac hypertrophy;
Ca2+
- From:
Chinese Circulation Journal
2014;(11):928-931
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of monocrotaline (MCT) on right ventricular function and expression of cardiac canonical transient receptor potential channels (TRPC) subfamily in experimental rats.
Methods: The SD male rats were randomly divided into 2 groups:Control group, the rats were normally fed and MCT group, the rats received a single dose injection of MCT 60 mg/kg to induce myocardial hypertrophy. n=10 in each group and all animals were treated for 3 weeks. The right ventricular hemodynamics parameters and right ventricular hypertrophy index (RVHI) were measured, right ventricular myocardium tissue section was observed by HE staining, the mRNA and protein expressions of TRPC subfamily were examined by RT-PCR and Western blot analysis.
Results: Compared with Control group, MCT group had increased RVSP, RVHI, RV+dp/dtmax and decreased RV-dp/dtmax, all P<0.01. In MCT group, the right ventricular myocardial cells had the thinker ifber, deeply stained nuclei with irregular shape. Compared with Control group, the mRNA and protein expressions of right ventricular TRPC6 were elevated in MCT group, n=6 and n=4 respectively, all P<0.05.
Conclusion:Right ventricular hypertrophy could be induced by 3 weeks MCT treatment, it up-regulating the mRNA and protein expressions of TRPC6 which might be involved in the occurrence and development of cardiac hypertrophy in experimental rats.
