Diabetes Reducing the Intensity of Sarcoplasmic Reticulum Ca2+-ATPase-SUMOylation of Myocardium in Experimental Rats
10.3969/j.issn.1000-3614.2014.11.020
- VernacularTitle:糖尿病减弱心肌组织肌浆网钙转运三磷酸腺苷酶的小泛素样修饰﹡
- Author:
Jing YAO
;
Xinghui SHAO
;
Guangyuan SONG
;
Zhenyan ZHAO
;
Haiyang GAO
;
Dewei WU
;
Siyong TENG
;
Yongjian WU
- Publication Type:Journal Article
- Keywords:
Diabetes;
Sarcoplasmic reticulum Ca2+-ATPase;
SUMOylation
- From:
Chinese Circulation Journal
2014;(11):932-936
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of diabetes on the intensity of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a)-SUMOylation and SERCA2a activity of myocardium in experimental rats.
Methods: The 8 weeks old SD rats were divided into 2 groups, Diabetic group, with diet-induced type 2 diabetic rats and Control group, with normal rats. The systolic and diastolic cardiac functions were evaluated by echocardiography and left ventricular pressure measurement. The intensity of SERCA2a-SUMOylation was examined by co-immunoprecipitation and SUMOylation kit.
Results: Compared with Control group, Diabetic group had decreased systolic and diastolic cardiac functions, especially for diastolic function;decreased SERCA2a protein expression and intensity of SUMOylation;decreased SUMOylation E2 (Ubc9 ) protein expression. The protein levels of SUMO1, SAE1 and SAE2 were similar between 2 groups.
Conclusion: The intensity of SERCA2a-SUMOylation and Ubc9 decreased in diabetic myocardium which implies that SERCA2a-SUMOylation and Ubc9 were closely related to the damage of diabetic myocardium in experimental rats.
