A rat model of chronic cerebral hypoperfusion for the research of revascularization
10.3760/cma.j.issn.1674-6554.2014.11.024
- VernacularTitle:一种适用于颅内外血管重建研究的大鼠慢性脑缺血模型
- Author:
Chao ZHANG
;
Xiaohong LI
;
Yue TU
;
Jianwei LI
;
Jingjing WANG
;
Shixiang CHENG
;
Sai ZHANG
;
Haiqian LIANG
- Publication Type:Journal Article
- Keywords:
Chronic cerebral hypoperfusion;
Rat models;
Cerebral revascularization;
Cerebral blood flow
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2014;(11):1041-1043
- CountryChina
- Language:Chinese
-
Abstract:
Objective To estimate whether the ligation of bilateral internal carotid artery in combination with one vertebral artery can lead to chronic cerebral hypoperfusion. Methods The sham operation, 2?VO and 3?VO rat models were subjected to the matching operation. Four weeks after operation,the cortical blood flow was determined. The learning and memory abilities were measured with Morris water maze test eight weeks later,then the rats were sacrificed to observe the morphological change of hippocampal CA1 region. Results Compared with the sham operation group((47±8.797)ml·min-1·100 g-1),the cerebral blood flow of 2?VO((24.30±8.999)ml ·min-1·100 g-1) and 3?VO((9.870±2.208)ml·min-1·100 g-1) were significantly decreased (P<0.01). Compared with the sham operation group((8.33±4.88)s),escape latencies of Morris water maze of 2?VO group ((14.78±7.84)s) and 3?VO group((14.86±7.96)s) in the fifth days also presented significantly increased (P<0.01),but rare difference between the two groups. Compared with the sham operation group[ (37.20±9.21) s, (10.01.±2.91)times],the target quadrant swimming time and crossing times of 2?VO group((20.13±5.80)s, (6.60±3.19)times) and 3?VO group((20.05±5.76)s,(6.55±2.59)times) in the fifth days also presented signifi?cantly decreased (P<0.01). There were distinct pathomorphology changes in hippocampal CA1 region of the two groups. Conclusion The ligation of bilateral internal carotid artery in combination with one vertebral artery can lead to chronic cerebral hypoperfusion,and can make the similar ethology representation with the 2?VO models.
