Establishment and significance of research-based clinical liver transplantation specimen bank for hepatocellular carcinoma
10.3969/j.issn.2095-4344.2014.49.020
- VernacularTitle:科研型肝癌肝移植临床标本库的建立及意义
- Author:
Qing ZHANG
;
Yuwen HAO
;
Yang YUE
;
Hong CHEN
;
Letian WANG
;
Xinguo CHEN
;
Zhongyang SHEN
- Publication Type:Journal Article
- Keywords:
liver neoplasms;
liver transplantation;
biological specimen banks
- From:
Chinese Journal of Tissue Engineering Research
2014;(49):7985-7989
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The establishment of a standardized clinical liver transplantation specimen bank is the primary condition for scientific research in this field, which can help to provide a qualified sample resource platform for research. OBJECTIVE:To primarily establish biological specimen bank of hepatocelular carcinoma for liver transplantation, to explore the standardized procedures of specimen colection, processing and preservation of hepatocelular carcinoma for liver transplantation, and to establish the sound and comprehensive information management system of clinical information of colected specimens. METHODS: In accordance with standardized procedures to establish biological specimen banks, the operational processes and quality control system were formulated. Liver tissue and blood samples of hepatocelular carcinoma recipients undergoing liver transplantation were regularly colected, managed and stored. Simultaneously, liver tissue and blood samples of benign liver disease in liver transplant recipients and of healthy donor were colected as controls. A systematic management was conducted in colected specimens and corresponding clinical information. RESULTS AND CONCLUSION:From August 2009, tissue and blood samples of 501 cases of receipts and donors undergoing liver transplantation with complete clinical information were colected from the specimen bank, including 203 hepatocelular carcinoma specimens, 214 benign liver disease specimens and 84 healthy donor specimens. These specimens included tumor tissue, adjacent tissues and distal non-cancerous tissue specimens, totaly 1 773. A total of 45 specimens were randomly selected for quality monitoring. The colected specimens had a high quality. Specimen information data computer management system was developed. This study initialy established a standardized research-based clinical transplantation specimen bank, which is helpful to elevate sample quality and has a good manipuility.
