A comparative study of HPV infection genotypes distribution in 308 cases of cervical intraepithelial neoplasia tissues
10.3969/j.issn.1673-4130.2014.23.011
- VernacularTitle:308例宫颈瘤组织中HPV感染基因型分布的对比研究
- Author:
Xiaojue ZHU
;
Jianxiang GENG
- Publication Type:Journal Article
- Keywords:
cervix;
tissue;
intraepithelial neoplasia;
human papillomavirus;
genotyping
- From:
International Journal of Laboratory Medicine
2014;(23):3180-3182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the distribution situation of human papillomavirus(HPV)genotypes in the tissue specimens of cervical intraepithelial neoplasia(CINⅠ,CINⅡ and CINⅢ)and its clinical significance.Methods The polymerase chain reaction (PCR)and gene-chips technology were utilized for detecting 23 kinds of HPV genotypes in the cervical tissue specimens from 53 ca-ses of CINⅠ,67 cases of CINⅡ and 188 cases of CINⅢ.And the related data of all subjects were analyzed.Results Among 53 ca-ses of CINⅠ,29 cases of HPV infection were detected with the total HPV infection rate of 54.72%9;among 67 cases of CINⅡ,52 cases of HPV infection were detected with the total HPV infection rate of 76.12%;among 188 cases of CINⅢ,175 cases of HPV infection were detected with the total HPV infection rate of 93.09%.Conclusion The significant difference of the total HPV infec-tion rate exists in the tissues of CINⅠ,CINⅡ and CINⅢ.PCR and the gene-chip technology can be applied in the detection of the cervical tissue specimens,23 kinds of genotypes can be detected by once detection,which has very important significance to the mo-lecular epidemiological survey of female cervical HPV infection,prevention and treatment of cervical cancer and the HPV vaccine re-search.
