Analysis on results of hepatitis C antibody detection by three kinds of ELISA reagents
10.3969/j.issn.1673-4130.2014.23.041
- VernacularTitle:3种酶联免疫吸附试验试剂检测丙型肝炎抗体结果分析
- Author:
Lijuan WU
- Publication Type:Journal Article
- Keywords:
hepatitis C virus;
double antigen sandwich method;
indirect method;
enzyme linked immunosorbent assay
- From:
International Journal of Laboratory Medicine
2014;(23):3246-3248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and evaluate the performance of the double antigen sandwich method reagents by comparing the detection results of three kinds of domestic hepatitis C virus(HCV)antibody detection kits with indirect method and sandwich method.Methods The different test methods were designed.The HCV antibody detection by using three kinds of reagents was performed on the collected positive and negative specimens and those specimens with positive reaction by the single and dual rea-gents were simultaneously performed the recombinant immunoblot assay(RIBA)and the nucleic acid detection;in addition,after the multiple proportion dilution,the quality control material with known content was parallelly detected with three kinds of reagents. All the detection results were conducted the comparison and the comprehensive analysis.Results Compared with the confirmation reagent,the false positive rates of two kinds of indirect method reagent and one kind of sandwich method reagent were 40.9%, 59.1% and 2.2% respectively.Their positive rates were 100%,but the analytical sensitivity of the sandwich method was higher than that of the indirect method by 1 ~4 times.Conclusion The sandwich ELISA reagent is significantly superior to the indirect EllSA reagent in the aspects of sensitivity and specificity.
