Effects of dietary interventionversus metformin treatment on retinol binding protein 4 expressions in rats with non-alcoholic fatty liver disease
10.3969/j.issn.2095-4344.2014.49.024
- VernacularTitle:非酒精性脂肪性肝病模型大鼠饮食或二甲双胍干预后肝视黄醇结合蛋白4表达
- Author:
Hong MA
;
Chunhua GUO
;
Xiangjiu YANG
;
Huadong LU
;
Ligang CHEN
;
Yanling HUANG
;
Wenqiang ZHANG
- Publication Type:Journal Article
- Keywords:
rat;
fatty liver;
retinol-binding proteins
- From:
Chinese Journal of Tissue Engineering Research
2014;(49):8007-8014
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Conflicting data have been reported regarding the expression of retinol-binding protein 4 in non-alcoholic fatty liver disease. OBJECTIVE:To evaluate the impact of dietary interventionversus metformin treatment on expression of retinol-binding protein 4 in rats with non-alcoholic fatty liver disease. METHODS: Fifty male Sprague-Dawley rats were randomized to six groups, including two normal control groups (rats were kiled after 8 and 16 weeks of normal diet), two HFD groups (rats were kiled after 8 and 16 weeks of high-fat diet), one dietary intervention group (rats were kiled after 8 weeks of high-fat diet and 8 weeks of normal diet) and one metformin treatment group (rats were kiled after 8 weeks of high-fat diet and 8 weeks of high-fat diet and metformin treatment). The levels of retinol-binding protein 4 in serum and biochemical indexes were detected through enzyme-linked immunosorbent assay. The expression of retinol-binding protein 4 mRNA in liver tissues was measuredvia western blot analysis, immunohistochemistry and RT-PCR. RESULTS AND CONCLUSION:Non-alcoholic fatty liver disease models were successfuly established by high-fat diet. Liver tissues of high-fat diet fed rats showed progressing non-alcoholic fatty liver disease histology, from non-alcoholic fatty liver to non-alcoholic steatohepatitis. Dietary intervention increased retinol-binding protein 4 expression in liver tissue as wel as improving liver enzyme, dyslipidemia and insulin resistance, and aleviated impaired liver histology. Metformin treatment only aleviated hepatic steatosis caused by high-fat diet. The results indicated that retinol-binding protein 4 expression might play a role in the pathogenesis of non-alcoholic fatty liver disease. Metformin treatment can aleviate non-alcoholic fatty liver disease histology,dietary intervention should be the fundamental treatment.
