Human umbilical cord mesenchymal stem cells co-culture ameliorates podocytic apoptosis：a possible role of HGF
10.3760/cma.j.issn.1001-7097.2014.12.009
- VernacularTitle:人脐带间充质干细胞共培养改善高糖诱导的足细胞凋亡
- Author:
Wenwen QI
;
Shasha LYU
;
Gang LIU
;
Jing CHENG
;
Yan SONG
;
Tongtong MING
;
Guangju GUAN
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Stem cells,mesenchymal;
Podocyte;
Apoptosis;
Hepatocyte growth factor
- From:
Chinese Journal of Nephrology
2014;(12):933-938
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of human umbilical cord mesenchymal stem cells (HUC?MSCs) on podocytic apoptosis and injury induced by high glucose (HG) and the underlying mechanisms. Methods Podocytes were divided into six groups according to treatment: ⑴ normal glucose group (NG);⑵high glucose group (HG);⑶mannitol control group (NG+Ma);⑷HUC?MSC co?culture group (HUC?MSCs); ⑸ recombinant human hepatocyte growth factor treatment group (rhHGF);⑹ neutralizing antibody group(HGF?NtAb). Cytometry and Hoechst staining were used to detect the apoptosis rates. Western blot was used to measure the ratio of active PARP to total PARP and the level of Bcl?2. Immunofluorescence was used to study podocytic apoptosis and injury. Neutralizing antibody (NtAb) was used to block its function and the recombinant cytokine was added to induce its function. Results High glucose induced podocytic apoptosis in a time?dependent manner, HUC?MSCs co?culture decreased the podocytic apoptosis rate and the expression of PARP (all P﹤0.05), increased the expression of Bcl?2, prevented the reduced expression and maintained the normal arrangement of podocytic podoplanin. The rhHGF prevented podocytic apoptosis and injury similarly to HUC?MSCs, the beneficial effect of HUC?MSC decreased when blockade of HGF. Conclusions HUC?MSCs co?culture ameliorates podocytic apoptosis and injure induced by HG, probably through secreting soluble HGF.
