The study of proteomics in acute spinal cord injury rats by iTRAQ technology
10.3969/j.issn.2095-4344.2014.49.004
- VernacularTitle:急性脊髓损伤模型大鼠蛋白质组学研究：iTRAQ技术的验证
- Author:
Jingjing BAI
;
Chong WANG
;
Liwen DING
;
Xinghua SONG
;
Jiangtao CHEN
;
Yijun ZHOU
;
Leilei XU
- Publication Type:Journal Article
- Keywords:
spinal cord injuries;
cerebrospinal fluid;
proteomics;
isotope labeling
- From:
Chinese Journal of Tissue Engineering Research
2014;(49):7891-7896
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Isobaric tags for relative and absolute quantitation (iTRAQ) mass spectrometry technology studys the information of relevant protein according to the ion signal shows different mass-to-charge ratio in the tandem mass spectrometry analysis. OBJECTIVE:To establish the protein spectrum of differential proteins in cerebrospinal fluid of acute spinal cord injury rat model, study the secondary injury mechanism and find an effective method of treating acute spinal cord injury from molecular level. METHODS:Acute spinal cord injury was produced in Sprague-Dawley rats and iTRAQ technology was applied to analyze the differential proteins in cerebrospinal fluid of acute spinal cord injury rat model. RESULTS AND CONCLUSION:Total 722 proteins have been identified in this study, including 107 differentialy expressed proteins, 63 downregulated proteins and 44 upregulated proteins. There were 19 proteins related to neurogenesis, including 14 up-regulation proteins and 5 down-regulation proteins. Seven proteins contributed to the regulation of neurogenesis. The differential proteins and growth factor identified in this study can be taken as the biomarkers of acute spinal cord injury or indicators of clinical monitoring of the progression, target treatment and efficacy assessment after acute spinal cord injury.
