Effect of HMGB1 on secretion of inflammatory cellular factor TNF-αand NO in mice macrophage infected with DEN2
10.3969/j.issn.1000-484X.2015.01.002
- VernacularTitle:重组HMGB1对DEN2感染Ana－1细胞分泌TNF－α及NO的影响
- Author:
Wei SUN
;
Zhengling SHANG
;
Li ZUO
;
Shiqi LONG
;
Qinghong MENG
;
Kun WANG
;
Ke YIN
- Publication Type:Journal Article
- Keywords:
DEN2;
HMGB1;
Ana-1
- From:
Chinese Journal of Immunology
2015;(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of different concentration HMGB1 on the secretion of TNF-αand NO from Ana-1 infected with DEN2 and virus copy.Methods:DEN2 were proliferated and identified by conventional methods.The adherence of DEN2 to Ana-1 was observed by direct immunofluorescence and RT-PCR.The level of virus mRNA were detected by qRT-PCR.The concentration of TNF-αwas detected by ELISA.The concentration of NO was detected with Griess reagent.Results:Ana-1 was able to adhered for DEN2.Compared with DEN group,the inhibition ratio(%) of the level of virus mRNA in D-HMGB1-1 group,D-HMGB1-10 group,D-HMGB1-100 group,D-HMGB1-1000 group was 41.53 ±2.12,55.30 ±1.59,74.75 ±1.12,86.35 ±1.42.Compared with DEN group,the level of TNF-αand NO decreased in D-HMGB1 groups(P<0.05).Conclusion:HMGB1 can be effectively regulated of Ana-1 secreted inflammation factor of infected with DEN2,and inhibited DEN2 replication.
