Membrane-expressed InsB15-23 H-2Kd dtSCT can reduce morbidity of type 1 diabetes mellitus in NOD mice
10.3969/j.issn.1000-484X.2015.01.010
- VernacularTitle:膜表达型InsB15－23 H－2 Kd dtSCT对NOD 小鼠1型糖尿病发病的影响
- Author:
Xingyuan PAN
;
Zedong CHEN
;
Yan DOU
;
Kai LIANG
;
Mingming YU
;
Mingchun JI
- Publication Type:Journal Article
- Keywords:
Disulfide-trap single-chain trimer;
CD8+T cells;
Type 1 diabetes mellitus;
NOD mice
- From:
Chinese Journal of Immunology
2015;(1):48-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To illuminate the influence of InsB15-23 H-2Kd dtSCT to the morbidity of type 1 diabetes mellitus in NOD mice.Methods:An eukaryotic plasmid encoded membrane-expressed InsB15-23 H-2Kd dtSCT was inoculated into 3 weeks old female NOD mice subcutaneously and the blood sugar and morbidity of type 1 diabetes mellitus were monitored once a week.To illuminate the cellular mechanism of immunologic intervention of membrane-expressed InsB15-23 H-2Kd dtSCT to the course of type 1 diabetes mellitus in NOD mice,the mononuclear cell infiltration of islets was detected by tissue slice and the frequency of IGRP206 2-14 specific CTLs in PBMC was analyzed by FACs.Results: As compared with pcDNA3.1 (-) control ( 60%) and untreated NOD mice ( 80%) , mice immunized with InsB15-23 H-2Kd dtSCT exhibited low level of islet infiltration and low morbidity in 30 weeks old ( 9%) .But the frequency of IGRP206-214 specific CTLs in PBMC of 16 and 40 weeks old mice showed no difference.Conclusion:Membrane-expressed InsB15-23 H-2Kd dtSCT can protect NOD mice from type 1 diabetes mellitus in IGRP206-214 independent pattern.
