Enhancement of epitope vaccines from W2b2a of Toxoplasma gondii potency using two kinds of immunomodulating agents in mice
10.3969/j.issn.1000-484X.2015.01.015
- VernacularTitle:两种免疫调节剂增强弓形虫W2b2a表位疫苗的小鼠保护作用
- Author:
Ronghua XIE
;
Kui TAN
;
Xiang WU
;
Hengping SHU
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
Pidotimod;
Thalidomid;
Epitope vaccine;
Immune effect
- From:
Chinese Journal of Immunology
2015;(1):73-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Pidotimod and Thalidomid to enhance the immune response and protective immunity induced by the epitope Vaccines From W2b2a of Toxoplasma gondii in mice.Methods:Mice were immunized intramuscularly with Pidotimod and pcDNA3-W2b2a,thalidomide and pcDNA3-W2b2a,respectively,then the immune response and the survival time of mouse attacked by Toxoplasma gondii were observed.Results:After the immunization,the level of IFN-γin sera of mice inculated with pcDNA3-W2b2a and Pidotimod,pcDNA3-W2b2a and Freund adjuvant,pcDNA3-W2b2a and Thalidomid were significantly higher than pcDNA3-W2b2a (all P<0.05).After the immunization,IgG,CD4+/CD8+T cell ratio ,proliferation of T cell induced by pcDNA3-W2b2a and Pidotimod,pcDNA3-W2b2a and Freund adjuvant were higher than pcDNA3-W2b2a,pcDNA3-W2b2a and thalidomid ( all P<0.05).After challenged with highly virulent tachyzoites,the mean survival time in immunized groups were significantly longer than control group (all P<0.05).Conclusion:Pidotimod ,Thalidomid adjuvant can increase protective immunity of epitope Vaccines From W2b2a of Toxoplasma gondii in mice.
