Effect of sinomenine on mouse RAW264.7 macrophage cells line polarization induced by LPS or IL-4
10.3969/j.issn.1000-484X.2015.01.012
- VernacularTitle:青藤碱对LPS、IL－4诱导的小鼠RAW264．7巨噬细胞极化的影响
- Author:
Jinfang LUO
;
Ruili ZHU
;
Lang YI
;
Yan DONG
;
Peixun WANG
- Publication Type:Journal Article
- Keywords:
Sinomenine;
Lipopolysaccharide;
Interleukin-4;
Macrophage polarization;
M1/M2 phenotype
- From:
Chinese Journal of Immunology
2015;(1):56-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate sinomenine (Sinomenine,SIN) effect on RAW264.7 cells polarization to M1 or M2 phenotype induced by lipopolysaccharide (LPS) or interleukin-4 (IL-4) .Methods:RAW264.7 cells were induced to polarize to M1 by LPS ,and to M2 by IL-4.Sinomenine effects on LPS or IL-4 induced macrophages:TNF-αand IL-10 secretion induced by different condition were detected by Enzyme linked immunosorbent assay (ELISA);The expression level of mRNA of Arginase1(Arg-1),Nitric oxide synthase(iNOS),suppressor of cytokine signaling protein-2(SOCS2) and suppressor of cytokine signaling protein-3(SOCS3) of M1/M2 phenotypes were detected by real time PCR respectively.Results:Sinomenine inhibited the increase of TNF-αsecretion,iNOS and SOCS3 mRNA expression level induced by LPS.Sinomenine inhibited the increase of IL-10 secretion and Arg-1 mRNA expression level induced by IL-4,but SOCS2 mRNA expression level was not affected by Sinomenine.Conclusion: Sinomenine can inhibite the macrophage polarization to M1 and M2 induced by LPS and IL-4.Sinomenine plays a regulatory role on imbalance of M1/M2,and is conducive to maintain the dynamic balance.
